Fig 4. cpr5-2 mutants are powdery mildew resistant.

Disease symptoms of wild type, cep1 and cpr5-2 single mutants and cep1 cpr5-2 double mutant plants upon leaf infection with E. cruciferarum were scored after visual inspection of the whole plant 10, 12 and 14 days post inoculation (dpi). Distribution of infected leaves in the three categories was carried out as described [18]. Infected leafs were distributed in the three categories <30%, 30–60%, and >60% diseased leaf area [18]. Columns of the same colour marked with different letters indicate statistically different groups (p < 0.05), and columns marked with identical letters indicate groups not statistically different (p > 0.05) according to the ANOVA- and Duncan test, and represent the frequency of plants distributed in the three categories of susceptibility. Data represent the respective means of eight experiments from independent inoculation events of the mutants with the corresponding parent background control. Each experiment comprised five plants per line.