Both male and female offspring of obese dams (OffOb) showed a significant higher level of Kiss1 mRNA in the posterodorsal medial amygdala (MePD) on pnd 30 compared with offspring of control dams (OffCon) (A and D). No significant difference was observed in Kiss1 mRNA expression in the hypothalamic arcuate (ARC) or anteroventral periventricular (AVPV) nuclei at this age (B, C, E, and F). In contrast, by pnd 100, maternal obesity reduced Kiss1 mRNA expression in the MePD of both males and females (A and D) and similarly reduced Kiss1 expression in the ARC and AVPV of female offspring (B and C), but not in the males (E, F). *p<0.05 vs OffCon, (n = 8–10 per group). Values are expressed as a ratio of Kiss1 to Hprt1 mRNA.