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. Author manuscript; available in PMC: 2018 Mar 8.
Published in final edited form as: Sci Transl Med. 2017 Mar 8;9(380):eaaf5347. doi: 10.1126/scitranslmed.aaf5347

Fig. 1. Automated glycan assembly of ST8 CPS frameshift oligosaccharides.

Fig. 1

(A) ST8 CPS structure and nomenclature of tetrasaccharide frameshifts A–D. The cellobiuronic acid (CA) moiety is highlighted. (B) Automated assembly of synthetic frameshifts A(1), B(2), C(3) and D(4) using resin 5 and building blocks 6–11. (C) Overlay of 1H NMR (600 MHz) spectra with commercially available ST8 CPS in D2O at 25 °C. Red arrowheads indicate peaks from pneumococcal C-polysaccharide as reported (18).