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. Author manuscript; available in PMC: 2018 Aug 10.
Published in final edited form as: Cell. 2017 Aug 10;170(4):649–663.e13. doi: 10.1016/j.cell.2017.07.023

Figure 6. Enhanced Energy Storage Can Correct Microglial Defects in TREM2-deficent 5XFAD Mice.

Figure 6

(A) TEM images of microglia sorted from 8-month-old 5XFAD, and Trem2−/− 5XFAD mice ± cyclocreatine.

(B) Quantification of the number of multivesicular and multilamellar structures/cell from A.

(C) Confocal images of brain sections of 8-month-old 5XFAD, and Trem2−/− 5XFAD mice ± cyclocreatine show Iba-1+ microglia (red), methoxy X04+ plaques (blue), and LC3 (green).

(D) Quantification of the number of LC3 puncta per HPF in the cortexes of the indicated mice.

(E) Quantification of the percentage of microglia that were cleaved caspase-3 positive from the indicated mice.

(F) Clustering analysis quantifying the number of microglia per mm3 within 15 μm of the surface of plaques.

*p<0.05, ***p<0.005 and ****p<0.001 by One-way ANOVA with Holm-Sidak’s multiple comparisons test (B, D–F) results pooled from 2 independent experiments representing a total of 5–8 male and female mice per treatment group. Arrowheads indicate multilamellar and multivesicular structures (A) or LC3+ vesicles (C). See also Figure S5.