Table 1. Average number of plasmid units per cell (N0) in starter cultures cultivated under antibiotic pressure.
Doubling time for each bacterial strain was determined using standard titer procedure.
| Plasmid | ||||||||
|---|---|---|---|---|---|---|---|---|
| None | pIB8 (R+M+cer+) |
pRB1 (R+M+cer−) |
pRB2 (R−M−cer−) |
|||||
| Doubling time (min.) | PCN (N0) |
Doubling time (min.) | PCN (N0) |
Doubling time (min.) | PCN (N0) |
Doubling time (min.) | ||
| E. coli strain | MG1655 (wt) |
21.3±2.4 | 14±2 | 19.5±4.0 | 16±3 | 20.8±2.4 | 15±3 | 19.1±2.6 |
| MG1655pcnB |
42.8±4.2 |
1.8±0.2 | 45.2±3.4 |
1.4±0.4 | 47.4±6.0 |
1.6±0.5 | 44.7±3.3 |
|
| JC8679 sbcA |
22.6±3.4 | 21±3.4 | 22.0±3.1 | 26.3±0.5 | 20.5±2.2 | 29.5±2.7 | 21.4±2.5 | |