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Average chemical shift changes in the 1HN and 15N resonances of XPA-MBD residues most affected by the binding of d9 plotted as a function of the molar ratio of DNA:protein. At a DNA:protein molar ratio of 2.5, the average chemical shift changes in the 1HN and 15N resonances of XPA-MBD are still increasing, indicating that a saturated state has not yet been reached.
