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. 2017 Aug 25;7:9473. doi: 10.1038/s41598-017-09958-9

Figure 2.

Figure 2

Murine UCBCs demonstrate long-term multi-lineage haematopoietic repopulating activity in adult transplantation setting. (A) Levels of donor chimerism [donor CD45 cells/(donor + host CD45 cells) × 100] were determined by flow cytometry in the haematopoietic organs of adult lethally-irradiated recipients at 1 month after the transplantation of 5 × 105 UCBCs (aUCBT) or BMCs (aBMT) (n = 4 aUCBT, n = 5 aBMT). *p ≤ 0.05 by Wilcoxon test. (B) Levels of chimerism analyzed serially in the PB of recipient mice between 1 and 12 months after the transplantation of 5 × 105, 2.5 × 105, or 1 × 105 UCBCs/mouse (each line in the plot represents a single mouse). (C) Representative lineage distribution of UCB-derived cells in the BM, spleen, and thymus of recipient mice at 4 months after aUCBT. Dot plots to determine donor-derived T cells (CD45.2+CD3+), B cells (CD45.2+B220+), myeloid cells (CD45.2+Mac-1+ and CD45.2+Gr-1+), and LSK cells (CD45.2+lineage-Sca-1+c-Kit+) are shown. (D) FACS sorting of CD45.2+ UCB-derived cells from the BM of a primary aUCBT recipient at 4 months after transplantation. (E) Representative photographs and count of the different subtypes of haematopoietic colonies on methylcellulose formed by UCB-derived (CD45.2+) BM sorted cells (10X magnification, bar: 400 μm). (F) Donor chimerism in the PB of secondary mice after the transplantation of 3 × 106 UCB-derived (CD45.2+) BM sorted cells (n = 3 recipient mice). Each black dot in the plot represents a single mouse, analyzed at 1, 2, and 4 months after transplant.