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. 2017 Aug 25;7:9502. doi: 10.1038/s41598-017-09858-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Partial amino acid alignment of PhoCHSA with selected CHS enzymes from other insects showing the conserved motifs found in all known insect CHS proteins. Identical residues are shown in dark grey background while conserved residues are shown in light grey background. M1 and M2: nucleotide binding sites similar to Walker A and Walker B motifs, M3 and M4: donor saccharide binding sites. M5: Acceptor binding site, M6: product binding site, M7: involved in chitin extrusion along with the 5TMS region (not shown). Aa: Aedes aegypti, Ag: Aphis glycines, Se: Spodoptera exigua, Tc: Tribolium castaneum, AaCHS1: AAEL002718-PA, AaCHS2: AAEL005618-PA, AgCHS: AFJ00066, SeCHSA: AAZ03545, SeCHSB: ABI96087, TcCHS1: NP_001034491, TcCHS2: NP_001034492.