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. 2017 Aug 25;7:9502. doi: 10.1038/s41598-017-09858-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Larval mortality after injection with three amounts (50, 100 and 200 ng) of three different dsRNA molecules; 5′-dsRNA, Mid-dsRNA and 3′-dsRNA targeting PhoCHSA mRNA. dsRNA was injected into 3^rd larval instars of potato tuber moth and larval mortality was recorded 120 hours post-injection. Error bars represent ±SE. Within the same dsRNA concentration different letters indicate significantly different values at P < 0.05.