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. 2017 Aug 25;7:9502. doi: 10.1038/s41598-017-09858-y

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effects of PhoCHSA RNA interference. Two-day-old 3rd instar larvae were injected with 200 ng of Mid dsRNA, 3′-dsRNA or 5′-dsRNA targeting PhoCHSA mRNA. Larval mortalities and abnormalities were detected at 120 hours post-injection. The figures represent larvae treated with 5′-dsRNA. Control larvae injected with dsRNA-free buffer show normal development (A). Some of the injected larvae are either died (black arrows) or show slower development of larvae (white arrows) (B) and others failed to pupate (C).