Table 1.
Nucleotide sequences of primers used in cloning, RACE, dsRNA synthesis and real time PCR analysis.
| Primer | Sequence 5′–3′ |
|---|---|
| Cloning primers | |
| CHSA201FD | CGSCGSGADGVVGGHAG |
| CHSA311RD | CTGTCCTGCTTCGGHGGRAWYTCYCGG |
| CHSA2529FD | GGYCATTGGCTGCARAAGGC |
| CHSA3072RD | GCGCCACCARTAACTGYA |
| CHSA4209FD | GCYATGTTGTTCCATMGATTYGAAAC |
| CHSA4440RD | CTCTTCTTCTGYTTNGCYTTYTC |
| CHSA214FS | GAAGCGATAACTCCGACGACGA |
| CHSA2679RS | CCAGCTATCCTCACCTTGATCGTACTG |
| CHSA1551RS | CCAAACCCAAGCCATTTGACGCGA |
| CHSA1345FS | GCTACATTTTTGGAAAGTTCGC |
| CHSA3207FS | GCGCTCTCAAGCTCATTCTT |
| CHSA4388RS | GTCGTTGTCGTAGTCGTCATCCAG |
| RACE primers | |
| 5′-end | |
| CHSA225RS | GGTGTGAGCTCGTCGTCGGA |
| CHSA216RS | TCGTCGTCGGAGTTATCGCT |
| 3′-end | |
| CHSA4202 FS | GTTCACTGCTATGTTGTTCCATCG |
| CHSA4365FS | CTGGATGACGACTACGACAACGAC |
| dsRNA primers | |
| CHSB-F 2177 | TAATACGACTCACTATAGGGCTCCATATGGAGGGAGACTT |
| CHSB-R 2663 | TAATACGACTCACTATAGGGTTCGTCAGATCGCAAAGTGT |
| CHSA-F 314 | TAATACGACTCACTATAGGGAGAAATCCCTCCGAAGCAGGACA |
| CHSA-R 834 | TAATACGACTCACTATAGGGCCGCGAGCTTCGAGCCAAAAGA |
| CHSA-F 3458 | TAATACGACTCACTATAGGG AGGATTCCTCCAAGGCGCGAAC |
| CHSA-R 3957 | TAATACGACTCACTATAGGGCCCTGGAAATACGAGCCTGTTCCT |
| AMPF | TAATACGACTCACTATAGGGCCCAGTGCTGCAATGATACC |
| AMPR | TAATACGACTCACTATAGGGTTCTGACAACGATCGGAGGAC |
| qRT-PCR primers | |
| CHSRTF-361 | GCCTGGAGTTCACAGTCAGA |
| CHSRTR-489 | GCCGGTCTTTCTTAAGTTGC |
| ACTF-135 | CGTSTTCCCSTCCATCGTVGG |
| ACTR-419 | ACATGATCTGSGTCATCTT |