Figure 1.

Mouse Neuro-2a and human SH-SY5Y cells are NeuN positive and express tyrosine hydroxylase (TH). Immunostaining was performed to show the expression of NeuN (red) and TH (green) in Neuro-2a cells (A) and SH-SY5Y cells (B). The cell nuclei were visualized by DAPI staining (blue) and cells were counted in 5 random fields at a magnification of 200x. Data were reported as the percentage of cells/field. Note that the majority of cells are both NeuN and TH positive. The size of SH-SY5Y cells on average is smaller than that of Neuro-2a cells, which together with their slower growth rate might explain the lower cell density of SH-SY5Y cells. Scale bar = 100 µm.