Figure 10.

Depletion of DDX3 enriches the binding of DNMTs and repressive histone marks to p53 promoter. (a–c) Schematic representation of p53 promoter containing CTCF-DNMT1-PARP1 complex binding sites in region p2-3 (from −628 to −259 bp)¹⁸ and p53 binding site in region p3 (from −165 to + 196 bp)¹⁵, respectively. DDX3 knockdown enhanced the binding abilities of DNMT1, DNMT3A, DNMT3B and repressive histone marks on p53 promoter and reduced the binding of p53 and active H3K4me3 histone mark. For chromatin immunoprecipitation assay, immunoprecipitates were analyzed by quantitative real-time PCR with specific primers for region p2 (a) p2-3 (b) and p3 (c) on p53 promoter. The relative binding activity of each protein on p53 promoter was normalized with input DNA and present as relative fold change against control rabbit IgG. Data are shown as average value ± S.D. calculated from at least two independent experiments. ***P < 0.001; **P < 0.01; *P < 0.05.