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. 2017 Aug 25;7:9411. doi: 10.1038/s41598-017-09779-w

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Overexpression of p53 rescues the centrosomal localization of DDX3 in p53 ^−/− HCT116 cells. (a). Representative confocal images demonstrate that centrosomal localization of DDX3 is restored after introduction of p53 wild-type (WT) or p53 S15A mutant (S15A) in p53 ^−/− HCT116 cells. The control, p53 WT- or p53 S15A mutant-overexpressed p53 ^−/− HCT116 cells were immunostained with anti-DDX3 (red), anti-γ-tubulin (green), anti-p53 (blue) antibodies and DNA (gray). Insets exhibit higher magnification of the centrosome. Scale bar = 5 μm. (b) Overexpression of p53 WT and p53 S15A mutant significantly enhances the percentage of γ-tubulin colocalizing with DDX3 in p53 ^−/− HCT116 cells. The colocalization of DDX3 and γ-tubulin was analyzed using Zeiss ZEN 2009 software and shown as means ± S.D. from three independent experiments. The value of 100 indicates perfect co-localization. ***P < 0.001. (n), the number of cells analyzed. (c) Western blot analysis showing the level of DDX3 and p53 in the control, p53 WT- and p53 S15A mutant-overexpressed p53 ^−/− HCT116 cells. GAPDH was used as an internal control. Original images of western blots were presented in Supplementary Fig. S5a.