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. 2017 Aug 25;7:9487. doi: 10.1038/s41598-017-10272-7

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Treatment of vEnv(X4) of J-Lat 6.3 T cells induces the transcriptional changes of multiple cellular genes involved in different signaling pathways. (A) Heatmap of genes (p < 0.05) in J-Lat 6.3 T cells untreated (n = 2) or treated with Env(X4)-VLP (n = 3) in technical replicates showing hierarchical clustering. (Lifescope v2.5.1 software from Life Technologies with the 2-mismatch setting). (B) Enrichment of genes modulated by vEnv. The fold enrichment was calculated based on the frequency of genes annotated to the term compared to their frequency in the genome in the DAVID Bioinformatics database.