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. 2017 Aug 25;7:9408. doi: 10.1038/s41598-017-09686-0

Figure 6.

Figure 6

Co-culture of vascular endothelial cells HUVECs with neuroblastoma microtumours from SY5Y in the static and perfusion culture. (a) Perfusion stabilised the micro-structure of neuroblastoma – endothelium co-culture over 14 days. Scale bar: 500 µm. (b) Perfusion increased the Tuj1 positively stained neurite structures in neuroblastoma SY5Y co-cultured with vascular endothelial cells HUVECs at day 35. Scale bar: 200 µm. Quantification of Tuji+ve fluorescent cell pretrusions by ImageJ shown in bar graph.