Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 25;7:9470. doi: 10.1038/s41598-017-09941-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Cell proliferation, viability and apoptosis studies. (A–D) Effect of rutin and vanillin on the viability of different cancer and normal cell lines: Cells were treated with increasing concentrations of rutin and vanillin (0–200 μM) for 48 h. Cell viabilities were shown as a percentage of the number of viable cells to that of the control. Values > 100 shows the percentage increase in cell survival to that of control (untreated cells). Each data point shown is the mean ± SD from n = 3. (E) Annexin-V staining of MCF-7 cells; cells were treated with IC₅₀ concentrations of rutin (80 μM) and vanillin (120 μM) for 48 h and subsequently stained with FITC-Annexin-V. Stimulation of apoptosis was quantified by flow cytometry. Representative flow images showing FITC-Annexin-V labeled cells, which directly corresponds to the percentage of apoptotic cells. (F) Bar graphs represents the percentage of apoptotic MCF-7 cells stained with Annexin-V for duplicate measurements ± SD. **p < 0.001, compared with the control (untreated cells). Statistical analysis was done using one-way ANOVA and t-test for unpaired samples. For anticancer activities doxorubicin has been taken as positive control.