Table 2.

Thermodynamic parameters obtained from the calorimetric titration of rutin and vanillin with MARK4.

Compound	K a (association constant), M−1	K D (dissociation constant) μM	ΔH (enthalpy change), cal/mol	ΔS, cal/mol/deg
Rutin	K a1 = 2.04 × 105 ± 0.15 × 105	K D1 = 4.9	ΔH 1 = −4.61 × 106	ΔS 1 = −1.52 × 104
	K a2 = 1.72 × 106 ± 0.31 × 106	K D2 = 0.581	ΔH 2 = −1.0 × 104	ΔS 2 = −4.74
Vanillin	K a1 = 8.43 × 103 ± 0.5 × 103	K D1 = 118.6	ΔH 1 = −4.90 × 105	ΔS 1 = −1.60 × 103
	K a2 = 1.45 × 105 ± 0.29 × 105	K D2 = 6.9	ΔH 2 = −1.22 × 104	ΔS 2 = −16.9 × 104