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. 2017 Aug 25;7:9528. doi: 10.1038/s41598-017-09910-x

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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DNA repair foci accumulate in differentiated PD63 cybrids. (A) Immunolocalization of γH2AX (red), ATM^pS1981 (p-ATM) (green) and 53BP1 (green) in PD63, PD67 and SH-SY5Y cells after differentiation. Cells were seeded and differentiated on coverslips and immunostained with specific antibodies. (B) The number of foci per nucleus in the three cell lines was quantified with CellProfiler as described in Material and Methods. Red lines indicate the means of γH2AX foci per nucleus. For these analyses more than 200 cells for each condition were analysed; **** = p < 0.0001. (C) Immunofluorescence showing the colocalisation of γH2AX (red) and ATM^pS1981 (p-ATM) (green) in differentiated PD63 cybrid cells. (D) Immunofluorescence showing the co-localization of γH2AX (red) and 53BP1 (green) in differentiated PD63 cybrid cells. Nuclei were stained by DAPI.