Figure 7.

GsCHX19.3 positively regulates saline-alkaline stress tolerance in plants. (A) Construction of GsCHX19.3 overexpression vector. (B) Characterization of GsCHX19.3 overexpression transgenic lines. WT was used as a control and Actin 2 was used as an internal standard. (C,D) Phenotypes of WT and OX seedlings under high salt (C), or carbonate alkaline stress (D). (E,F) Primary roots of WT and OX seedlings under high salt (E), or carbonate alkaline stress (F). The 11-day-old seedlings grown on normal 1/2MS medium were transferred to new plates supplemented with 0, 100, 115 mM NaCl or 0, 8, 9 mM NaHCO₃. Photographs were taken after 7 days. Fifteen seedlings of each line were used for each experiment. (G) Phenotypes of WT and OX adult seedlings under carbonate alkaline. (H) Survival rates of WT and OX adult seedlings under carbonate alkaline. The 4-week-old soil-grown seedlings were watered with 150 mM NaHCO₃ (pH 9.0) solution. Photographs were taken after 15 days. Data are means (±SE) of three replicates (P < 0.05, one-way ANOVA).