Table 1.
Expression of transcriptions related to different collagens, collagen maturation, non‐collagenous ECM components, ECM remodelling and select ECM receptors, by hPCLS and chPCLS after treatment with 10 μM galunisertib for 48 h. LDA results are expressed as average fold induction ± SEM relative to GAPDH (n = 4)
| Gene | hPCLS | chPCLS |
|---|---|---|
| Type of collagen | ||
| COL1A1 | ↓ 0.13 ± 0.03* | ↓ 0.13 ± 0.02* |
| COL1A2 | ↓ 0.40 ± 0.12* | ↓ 0.26 ± 0.09* |
| COL3A1 | ↓ 0.46 ± 0.01* | ↓ 0.33 ± 0.06* |
| COL4A1 | ↓ 0.31 ± 0.04* | ↓ 0.28 ± 0.03* |
| COL5A1 | ↓ 0.37 ± 0.10* | ↓ 0.20 ± 0.03* |
| COL6A1 | ↓ 0.67 ± 0.07* | ↓ 0.63 ± 0.14 |
| Collagen maturation | ||
| PLOD1 | ↓ 0.54 ± 0.07* | ↓ 0.75 ± 0.15 |
| PLOD2 | ↓ 0.54 ± 0.22 | ↓ 0.49 ± 0.15 |
| PLOD3 | ↓ 0.87 ± 0.17 | ↑ 1.12 ± 0.31 |
| P4HA1 | ↑ 1.16 ± 0.26 | ↔ 0.95 ± 0.43 |
| P4HA2 | ↔ 1.02 ± 0.37 | ↑ 1.45 ± 0.18 |
| P4HA3 | ↓ 0.47 ± 0.13 | ↓ 0.22 ± 0.04* |
| P4HB | ↓ 0.78 ± 0.08 | ↑ 1.17 ± 0.34 |
| LEPRE1 | ↓ 0.75 ± 0.09 | ↓ 0.55 ± 0.12 |
| LEPREL1 | ↓ 0.71 ± 0.13 | ↓ 0.51 ± 0.10* |
| LEPREL2 | ↓ 0.60 ± 0.15 | ↓ 0.82 ± 0.32 |
| LOX | ↔ 0.91 ± 0.58 | ↓ 0.51 ± 0.15 |
| LOXL1 | ↓ 0.42 ± 0.13* | ↓ 0.62 ± 0.19 |
| LOXL2 | ↓ 0.49 ± 0.06* | ↓ 0.34 ± 0.05* |
| LOXL3 | ↓ 0.81 ± 0.32 | ↓ 0.55 ± 0.16 |
| LOXL4 | ↓ 0.40 ± 0.16 | ↓ 0.31 ± 0.09* |
| SERPINH | ↓ 0.59 ± 0.12 | ↓ 0.43 ± 0.04* |
| ADAMTS2 | ↓ 0.70 ± 0.14 | ↓ 0.47 ± 0.16 |
| ADAMTS3 | ↑ 1.97 ± 1.44 | ↓ 0.42 ± 0.09* |
| ADAMTS14 | No expression | ↓ 0.43 ± 0.17 |
| BMP1 | ↓ 0.71 ± 0.07* | ↓ 0.40 ± 0.07* |
| PCOLCE | ↓ 0.75 ± 0.06* | ↓ 0.30 ± 0.04* |
| PCOLCE2 | ↓ 0.83 ± 0.15 | ↓ 0.84 ± 0.44 |
| FKBP10 | ↓ 0.47 ± 0.04* | ↓ 0.38 ± 0.05* |
| SLC39A13 | ↔ 0.91 ± 0.08 | ↓ 0.79 ± 0.10 |
| COLGALT1 | ↓ 0.78 ± 0.12 | ↓ 0.67 ± 0.14 |
| Extracellular matrix component | ||
| Fibronectin 1 | ↓ 0.13 ± 0.03* | ↓ 0.13 ± 0.02* |
| Elastin | ↓ 0.40 ± 0.12* | ↓ 0.26 ± 0.09* |
| Decorin | ↓ 0.46 ± 0.01* | ↓ 0.33 ± 0.06* |
| Biglycan | ↓ 0.31 ± 0.04* | ↓ 0.28 ± 0.03* |
| Fibromodulin | ↓ 0.37 ± 0.10* | ↓ 0.20 ± 0.03* |
| Extracellular matrix remodelling | ||
| MMP1 | ↑ 1.31 ± 0.44 | ↑ 1.95 ± 0.72 |
| MMP13 | No expression | ↑ 6.37 ± 1.47* |
| MMP14 | ↓ 0.42 ± 0.02* | ↓ 0.38 ± 0.05* |
| TIMP1 | ↓ 0.47 ± 0.03* | ↓ 0.40 ± 0.10* |
| CTSK | ↓ 0.70 ± 0.10 | ↓ 0.62 ± 0.15 |
| Extracellular matrix protein receptor | ||
| DDR1 | ↓ 0.65 ± 0.05* | ↓ 0.55 ± 0.07* |
| DDR2 | ↔ 1.00 ± 0.21 | ↓ 0.42 ± 0.07* |
| MRC2 | ↓ 0.24 ± 0.07* | ↓ 0.15 ± 0.02* |
↓ indicates down‐regulation (fold induction < 0.9).
↑ indicates up‐regulation (fold induction > 0.9).
↔ indicates no difference (0.9 ≤ fold induction ≤ 1.1).
*
P < 0.05, significantly different from control.