Figure 5.

Cholesterol efflux impairment in IFNγ‐treated foam THP‐1 cells is prevented by tofacitinib via its effect on ABCA1. (A) Quantification of Oil Red‐O positive staining in THP‐1 cells (vehicle‐ or ABCA1 siRNA‐treated) in the presence or absence of HFD rabbit serum, 50 ng·mL⁻¹ IFNγ and 2 μM tofacitinib for 24 h and after a 6 h incubation period with the efflux medium.* P < 0.05 versus vehicle, # P < 0.05 versus basal media, $ P < 0.05 versus HFD rabbit serum and & P < 0.05 versus HFD rabbit serum + IFNγ. Bars show the mean and SEM (n = 5). (B) Representative images of THP‐1 cells stained with Oil Red‐O. Scale bar = 10 μm. (C) Representative image of ABCA1 protein expression assessed by Western blot in THP‐1 cells at the end of the experiment.