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. 2017 Aug 21;8:1458. doi: 10.3389/fpls.2017.01458

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Copyright © 2017 Li, Wang, Xu, Li, Sun, Guo and Ji.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SUMOylation in soybean root is induced by stress. Fifteen microgram of total proteins extracts from the soybean roots at the second trifoliolate leaf stage were subjected to western blot analysis against anti-AtSUMO1. Coomassie stained of high abundant protein showed equal loading of protein samples. (A) NaCl (200 mM) (B) Heat stress (42°C) (C) ABA (100 μM). Free SUMO and conjugates are highlighted by the arrowheads and brackets respectively. The asterisk denotes a non-inducible immunoreactive product.