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. Author manuscript; available in PMC: 2018 Feb 1.
Published in final edited form as: Anal Chem. 2017 Jul 20;89(15):7852–7860. doi: 10.1021/acs.analchem.6b04194

Figure 4.

Figure 4

The effect of ubistatin or phosphorylation of Ub at S65 on E1 and E2-25K catalyzed Ub chain formation. SDS-PAGE gels of in vitro E1 and E2-25K-catalyzed polymerization reactions for UbWT (A and left panel in C) or equimolar mixture of UbWT with ubistatin A (B) or p-S65-Ub (right panel in C).