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. Author manuscript; available in PMC: 2018 Oct 1.
Published in final edited form as: J Immunol Methods. 2017 Jun 22;449:15–22. doi: 10.1016/j.jim.2017.06.009

Figure 4. Comparison of surface marker-based suppression assay and CFSE-based suppression assay in the presence of Pam3CSK4.

Figure 4

(a) Percentage suppression of CD25+CD134+ Teff at day 2 in the absence and presence of Pam3CSK4. The median frequency of CD25+CD134+ on day 2 in Teff cultured without Pam3CSK4 was 57% (range 30–82%). (b) Percentage suppression of CD25+ Teff at day 4 in the absence and presence of Pam3CSK4. (c) Percentage suppression of CFSE-based proliferation at day 4 in the absence and presence of Pam3CSK4. Wilcoxon test of paired data: (a) *P=0.011, (b) ***P=0.001, and (c) **P=0.002. Data from 13 healthy controls are shown with cells at Tregs:Teff ratios of 1:4.