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. Author manuscript; available in PMC: 2018 Sep 1.
Published in final edited form as: Am J Transplant. 2017 Apr 18;17(9):2326–2337. doi: 10.1111/ajt.14266

Figure 2. Atg5 plays a key role in survival of DC under IRI.

Figure 2

A) Splenic DCs were isolated from WT and CD11cCre/Atg5 conditional knockout mice. DCs were stained for Annexin-V and 7-AAD after 20 hours of exposure to 5μM or 10μM of H2O2. Double negative cells were labeled as live. Atg5 deficient DCs showed significantly lower survival rates as compared to WT DCs. B) Ischemic WT or Atg5 conditional knockout hearts were transplanted into BALB/cJ recipients. Grafts were then harvested 1 day post-transplant. H2-kd negative (donor) DCs were studied for rate of death. Ischemic Atg5 deficient DCs showed significantly higher apoptosis as compared to WT donor DCs (Right upper quadrant) (n=3/group, *p<0.05).