Trex1 and Aim2 double knockdown induces a type I IFN response in macrophages. IFN-β, CXCL10, Trex1, and Aim2 RNA levels upon knockdown of the genes indicated in NR9456 cells (A) and BMDMs (B). RNA were analyzed by RT-qPCR. The values presented are normalized to Gapdh and are the mean ± the standard error of the mean of three experiments. *, P < 0.05; **, P < 0.005; ***, P < 0.0005 (two-tailed t test). The knockdown of TREX1 and AIM2 protein levels by siRNA treatment is shown in Fig. S2B. siCont, control siRNA.