Fig. 5.

Proliferation of Aha1^−/− null mutants and control strains under stress. L. donovani cells (1 × 10⁵ mL⁻¹) were seeded into 10 mL of supplemented M199 and grown for 4 days. The y-axis shows the cell density (×10⁷). Parasites were grown at 25 °C and pH 7.0 (n = 14) (a), 37 °C and pH 7.0 (b) and 25 °C and pH 5.5 (c). Additional cultures were grown as in a with the addition of 2% ethanol (=IC₅₀) (d). n = 8; *p ≤ 0.05; **p ≤ 0.01; ***p ≤ 0.001 (paired Student’s t test)