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. 2001 Jun 15;29(12):2535–2541. doi: 10.1093/nar/29.12.2535

Figure 5.

Figure 5

Primer extension on UV-crosslinked 249 base RNA. (Left) PAGE of primer extension reactions using the 249 base RNA and primer C65. Left lanes contain dideoxynucleotides causing stops to occur at the indicated template bases: A, G, C and U. Lane X contains the gel-purified, UV-crosslinked 249 base RNA. The arrow on the right indicates that primer extension stops at base A57. Lane ‘No UV’ contains gel-purified 249 base RNA that has not been exposed to UV light. (Right) Lanes A, G, C and U indicate the sequence of the 249 base RNA template using primer C128. Lane X contains the gel-purified, UV-crosslinked 249 base RNA. The arrow on the right indicates a stop at base G107. Lane ‘No UV’ contains a similar reaction using 249 base control RNA as template.