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. 2017 Aug 24;8:1013. doi: 10.3389/fimmu.2017.01013

Figure 6.

Figure 6

Downregulation of LILRB4 on decidual macrophages by Toxoplasma gondii infection changed the expression of the arginine catabolism enzymes type-I arginase (Arg-I) and inducible nitric oxide synthase (iNOS). (A) Representative depictions of iNOS (131 kDa) and Arg-I (35 kDa) protein levels in uninfected, infected, and LILRB4-neutralized and infected human decidual macrophages by western blot. (B) Histograms analysis of western blot for iNOS and Arg-I expression in uninfected, infected, and LILRB4-neutralized and infected human decidual macrophages. Representative histograms for iNOS (C,D) and Arg-I (E,F) protein levels in uninfected, infected, and LILRB4−/− infected mouse-decidual macrophages by flow cytometry. Representative data for in vivo analysis of different individuals in uninfected, infected, and LILRB4−/− infected groups. Representative data for in vitro analysis of one individual from uninfected, infected and LILRB4-neutralized and infected groups. Data are expressed as means ± SD of 10 pregnant mice or 9 human spontaneous abortion samples for each treatment group; *p < 0.05, **p < 0.01, by unpaired t-test.