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. 2017 Aug 24;8:1441. doi: 10.3389/fpls.2017.01441

FIGURE 1.

FIGURE 1

Schematic of RNA-templated DNA Repair. Pol III transcribed RNA was used for the gRNA. (A) In cis repair, the gRNA (blue) is attached directly to the repair template (green). (B) In trans repair, a tRNA (red) separates the gRNA and the repair template, which are processed into two separate RNA molecules; arrows indicate the cleavage positions. Below is a schematic of cis and trans repair at a target locus, where a target-specific gRNA produces a CRISPR/Cas9-mediated DSB that is repaired by HDR. The repair template is in antisense or sense orientation relative to the gRNA sequence. Dark blue line, 20-nt target specific gRNA; light blue line, scaffold RNA; green line, repair template; the small vertical line represents the nucleotide sequence, the green vertical lines represent the PAM sequence, the red vertical lines represent mutations introduced through template-mediated repair.