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. 2017 Aug 28;7:9655. doi: 10.1038/s41598-017-10189-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparing effects of introducing miR-34b-5p and miR-34c-5p mimics into MDA-MB-231 cells. Cells were transiently transfected with mimics representing miR-34b or miR-34c or a negative control mimic. (a) Venn diagram showing the number of significant differentially expressed transcripts (q ≤ 0.05) 48 h after transfection with miR-34 mimics. (b) Enriched biological processes based on transcript level changes following introduction of miR-34b (blue bars) or miR-34c (red bars), as determined by Ingenuity Pathway Analysis. (c) Cellular growth rate is shown as cell density versus time. One representative experiment is shown (n = 7). Error bars represent standard error of mean (SEM) for technical triplets. (d) Apoptosis is shown as number of caspase 3/7-positive cells per well versus time. One representative experiment is shown (n = 5). Error bars represent SEM for technical triplets. (e) Phase-contrast images of cells 72 hours after introduction of mimics, magnification 10x. The arrow indicates cells with changed morphology in miR-34c transfected cells. (f) The migratory capacity of the transfected cells was determined using a transwell pore assay (n = 2). Data is shown relative to control-transfected cells. Error bars represent SEM for biological experiments.