Fig. 3.

LTβR expression by Ccl19 ^−cre-positive stromal cells is required for mLN lymphangiogenesis. Ccl19 ^−cre × LTβR ^fl/fl (LTβR ^fl/fl) and wild-type littermate control Ccl19 ^−cre × LTβR ^+/+ (LTβR ^+/+) mice were infected with Hp and the entire chain of the mLN collected at the indicated time-points. a mLN serial cryosections showing lymphatics in naive mice or at 12 and 21 dpi (blue; DAPI, red; LYVE-1⁺). Scale bar = 2000 μm. Images are from single mice and are representative of two independent experiments each including n ≥ 2–3 mice/group/time point. b Quantitation of the mLN area occupied by LYVE-1⁺ LECs in naive and Hp-infected animals. Data represents mean ± SEM and are pooled from two independent experiments with each dot representing an individual mouse. c mLN serial cryosections showing LYVE-1⁺ stromal cell organization and DC distribution within the mLN at 21 dpi (Cyan; CD11c and red; LYVE-1⁺). Scale bar = 2000 μm. d Quantitation of the mLN area occupied by DCs (CD11c⁺) in infected LTβR ^+/+ and LTβR ^fl/fl mice. Data represents mean ± SEM and are pooled from two independent experiments with each dot representing an individual mouse. Statistical analyses were performed using ANOVA, Bonferroni’s multiple comparison test and significance donated as *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001