Fig. 4.

B cells provide the lymphotoxin required to drive mLN lymphangiogenesis. Mixed bone marrow chimeras were generated as described in Methods section. Chimeras lacking lymphotoxin expression exclusively on B cells (Jht ^−/− +LTβ ^−/−) or T cells (TCRδβ ^−/− +LTβ ^−/−) were compared to control mice receiving B-cell or T-cell deficient bone marrow mixed with WT cells (Jht ^−/− +WT and TCRδβ ^−/−+WT). All mice were infected with Hp and the entire chain of the mLN collected at day 0 (naive) and 21 dpi. a, b mLN cryosections from infected chimeric mice lacking lymphotoxin on B cells (Jht ^−/− +LTβ ^−/−) and their respective controls (Jht ^−/−+WT) showing immunofluorescence images staining for LYVE-1⁺ (red) LECs network. c, d mLN cryosections from mixed BMC infected mice lacking lymphotoxin on T cells (TCRδβ ^−/− +LTβ ^−/−) and respective controls TCRδβ ^−/−+WT) showing immunofluorescence images staining for LYVE-1⁺ (red) LECs network. Scale bar = 2000 μm