. 2017 Aug 28;4(1):39. doi: 10.1186/s40643-017-0169-1

Table 1.

Effects of metal ions on the enzymatic activity of the recombinant AcCR

Reagent	Concentration (mM)	Relative activity (%)
Control		100.0 ± 0.8
Mn²⁺	2	122.3 ± 0.4
Mn²⁺	5	119.9 ± 0.7
K⁺	2	110.6 ± 1.2
K⁺	5	103.4 ± 0.8
Fe²⁺	2	106.7 ± 1.0
Fe²⁺	5	106.0 ± 2.9
Zn²⁺	2	94.8 ± 1.2
Zn²⁺	5	85.8 ± 1.3
Mg²⁺	2	115.7 ± 0.7
Mg²⁺	5	123.5 ± 2.4
Ba²⁺	2	100.8 ± 0.5
Ba²⁺	5	97.9 ± 1.7
Ca²⁺	2	116.7 ± 0.6
Ca²⁺	5	113.9 ± 1.0
Cu²⁺	2	43.0 ± 0.7
Cu²⁺	5	24.9 ± 0.7
Co²⁺	2	99.6 ± 2.1
Co²⁺	5	113.8 ± 0.2
Hg²⁺	2	Nd
Hg²⁺	5	Nd
Ag⁺	2	Nd
Ag⁺	5	Nd

Reaction conditions: 0.5 mM NADH, 2 mL 50 mM citrate–phosphate buffer (pH 6.5) with different metal ions, and 5 mM 4′-chloroacetophenone were incubated for 10 min at 35 °C before adding 20 μL purified recombinant AcCR (about 0.008 mg of the purified enzyme), and recorded the changes of absorbance for 3 min