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Kinetic parameters of different substrates for the recombinant AcCR
| Substrate | K m (mM) | V max (μmol min−1 mg−1) | k cat (min−1) |
|---|---|---|---|
| NADH | 0.16 | 5.19 | 145.7 |
| 4′-Chloroacetophenone | 0.26 | ||
| NADPH | 0.44 | 0.66 | 17.71 |
| 4′-Chloroacetophenone | 2.75 | ||
| NAD+ | 0.38 | 11.8 | 265.8 |
| Isopropanol | 23.8 | ||
| NADP+ | 1.39 | 1.32 | 35.94 |
| Isopropanol | 54.1 |
