Table 2.
Locus-Specific Primer Sequences for MiSeq Deep Sequencing
| Locus Name | 5′-3′ Forward Primera | 5′-3′ Reverse Primerb |
|---|---|---|
| USH2A (on-target) | AGGGCTTAGGTGTGATCATTGC | TAGCATTACAGACAGTCCCAGG |
| STK3 | TCATGCTCAGTGCACTGTAC | CTCTGTTCACAGATGATATG |
| FHIT | CTTCTCTTGACTAGGAAAGG | CCACCATTTAAAAGCCTCCC |
| TENM2 | TTGCACTTCCCAGATGAGGTG | GGATATGAACTTTCTCCAACAC |
| ROR1 | CAGAATTCCGATTTCTTGTCTC | GCCCACCATTATAACATTTCAG |
| SPG20 | GCAAAGATATAATGGACATGG | TGACAGATAATCTTCTGATCC |
| TBR1 | TGCTGGTGCCCTTTTCTTTAGG | TGTCTCAGGCGCTCAATGTAAG |
| DMD | AGAGGAACACACCAAATCTGG | AGCCACGTATTTGTCTTTCC |
| RGS7BP | CAGTGTTCTCAATGTCTGTGG | TGACTCAAAGCATAGCCAACC |
| EHD1 | CCTCTTAGTGTGTGCTTTGG | AGTGGATGCTTCAGTTGCTG |
| KLHL15 | ACATAAATTGCAGGACATGGC | AGGATTCTGTTCCTCCATCC |
| Intergenic | GTGCATACAAGAGTGGCTTG | ACCCTTCTGGATGAAAGCTG |
| TIM22 | TGGTCTTCTCGGCAGAGATC | AAGCTCCGCTGCAGTACAGC |
| HNRNPUL2 | CTCCATTTCTCTTCCAGTGG | TGCGACACATCTTAGCTCG |
| C5orf66 | AGTCTCTGAGAGGCTTTGGATG | ACAGCTTGGGTGTTCAGAAACC |
| RBFOX1 | CTAAGTGCACTACATGGTCACC | ACTGCCAAGAAGAGCTTCTTCC |
| LOC730100 | AAAGATTGACCTCAGAGCC | GTAAATATGCTGCAGAGAC |
| IGKV1-33 | TACAACTGCCAATCATGTGGTC | AGAAACCCTATGCCTTCTCTGG |
| IGKV1D-33 | ATAGAGAAGGTACAACTGCC | CAGAAACCCTATGCCTTCTC |
| BAZ2B | TGCTAAGGTAATCAAAGTG | CTTTGAGAGAGTTTCACTC |
| TANC2 | TGTGAGAATCCTCCACTTTGG | ATGAGGTAGCAGTGAACTGTG |
| SLX4IP | GGAAATTCTCATATGGCTGG | GCTATTCCCAACAGTTTGTG |
a
Forward primers have appended the last 5′-3′ 34 bases of the TruSeq adaptor sequence: TACACTCTTTCCCTACACGACGCTCTTCCGATCT.
b
Reverse primers have appended the first 3′-5′ 34 bases of the TruSeq adaptor sequence: GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT.