Figure 2.

Apoptosis Was Not the Main Cause for MYXV Infection-Associated Cell Death in OC Tumor Cells

SKOV3, OvCa-26, and OvCa-2a cells were mock treated, infected with either vMyxGFP (WT) or vMyxM062RKO at an MOI of 5 for 24 hr before being stained with Annexin V and propidium iodide (PI) for flow cytometry. Cells from the infection group were gated first with forward scatter to side scatterplot (FSC/SSC) followed by the GFP-positive population (A) to analyze the Annexin V-PI staining. Next, GFP-negative cells were gated (B) to analyze the Annexin V-PI staining. This is a representative view of three independent experiments. In the quadrants shown, x and y axes indicate fluorescence intensity, while quadrants of each cell line were defined with the use of mock-treated samples, respectively.