Figure 3.

MYXV Treatment Complements Chemotherapy in a Replication-Independent Manner against Primary OC Tumor Cells

(A) MYXV complements gemcitabine for improved cytotoxicity. Primary patient-ascites-derived OvCa-2a tumor cells were mock-treated or infected with MYXV (WT or M062R null MYXV) at an MOI of 10; gemcitabine treatment at the given doses followed, or pretreatment with gemcitabine was given before viral infection. The first treatment (virus or chemotherapy) lasted 48 hr, followed by 24 hr of growth in fresh medium; cells were then treated with a second treatment (chemotherapy or virus) for another 48 hr before cell viability was measured. (B) MYXV complements cisplatin-associated cytotoxicity. The primary OvCa-26 OC cell line was used in this study, together with cisplatin. The study was conducted similarly to that with gemcitabine as described above. The error bar represents the SEM, and the mean is calculated from quantification in triplicate. Shown is a representative of two independent experiments.