Table 1.
Primary Antibodies used in the study.
| Protein | Source | Clone/Cat. No. | Species | Immunogen | Dilution |
|---|---|---|---|---|---|
| 8-Hydroxy-2′-deoxyguanosine | Abcam | Cat# ab48508 | Mouse | 8-Hydroxy-2′-deoxyguanosine conjugated Keyhole Limpet Hemocyanin | 1:20,000 |
| Actin | Sigma | Clone AC-15 | Mouse | Slightly modified β-cytoplasmic actin N-terminal peptide | 1:20,000W |
| APP | *C Masters | Clone 22C11 | Mouse | Purified recombinant Alzheimer precursor A4 (pre A4695) fusion protein. | 1:1,500 |
| Ceruloplasmin | Dako | Cat# Q0121 | Rabbit | Ceruloplasmin isolated from human plasma | 1:10,000W |
| cFOS | Santa-Cruz | Cat# sc-253 | Rabbit | Epitope mapping within an internal region of c-Fos of human origin | 1:5,000 |
| CRALBP | Abcam | Cat# ab15051 | Mouse | Recombinant full length human CRALBP | 1:2,000 |
| gp91phox | BD Biosciences | Clone 53/gp91[phox] | Mouse | Mouse gp91[phox] aa. 450–556 | 1:500 |
| HO-1 | Enzo Life Sciences | Cat# SPA-895 | Rabbit | Recombinant rat HO-1 (Hsp32) lacking the membrane spanning region | 1:2,500, 1:2,000W |
| Interleukin-6 | R&D Systems | Cat# AF506 | Goat | E. coli-derived recombinant rat IL-6 Phe25-Thr211 | 1:500 |
| Iba1 | Wako | Cat# 019-19741 | Rabbit | Synthetic peptide corresponding to the Iba1carboxy-terminal sequence | 1:4,000f |
| LDH-A | Santa-Cruz | Cat# sc-27230 | Goat | Epitope mapping at the N-terminus of LDH-A of human origin | 1:2,000 |
| Myelin basic protein | Dako | Cat# A0623 | Rabbit | Myelin basic, protein isolated from human brain | 1:3,000 |
| p67phox | BD Biosciences | Clone 29/p67phox | Mouse | Human p67 [phox] aa. 317-469 | 1:1,000W |
| p-cJUN | CST | Cat# 3270 | Rabbit | Synthetic phosphopeptide corresponding to residues around Ser73 of human c-Jun | 1:5,000 |
| Pimonidazole | Hypoxyprobe Inc | Clone 4.3.11.3 | Mouse | Pimonidazole adducts | 1:500 |
| SOD-1 | Calbiochem | Clone 6F5 | Mouse | Purified recombinant fragment of human SOD1 expressed in E. Coli | 1:2,500 |
| SOD-2 | Antibody Technology Australia Pty Ltd | Cat# SOD2R | Rabbit | Human/rat/mouse SOD2 aa. 25–43 | 1:2,500 |
| Transferrin receptor | ThermoFisher | Clone H68.4 | Mouse | Recombinant human transferrin receptor | 1:500 |
| Vimentin | Dako | Clone V9 | Mouse | Purified vimentin from porcine eye lens | 1:200f |
f
Dilution used for 2-step fluorescent immunostaining procedure;
W
Dilution used for Western blotting;
*
Gifted by C Masters, University of Melbourne.