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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Bioessays. 2017 Jun 16;39(8):10.1002/bies.201700025. doi: 10.1002/bies.201700025

Figure 2.

Figure 2

Model of break-induced replication. A) A single-ended DSB leads to BIR. A break in the lagging strand template is shown for simplicity, but other causes of fork collapse or nuclease cleavage (e.g. HO endonuclease [68] and camptothecin inhibition of topoisomerase I [85], have been used to produce seDSB. B) Displacement of the lagging strand template allows leading strand ligation to form an intact chromatid. The seDSB is subject to 5′ end resection and RPA binding. C) RPA is replaced by RAD51 to form and invading (acceptor) filament. The acceptor DNA released by branch migration of the unstable D-loop is a template for lagging strand conservative DNA synthesis.