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Mi2 is a DNA-dependent, nucleosome-stimulated ATPase. (A) Silver-stained SDS–PAGE containing the NuRD complex and recombinant Mi2. The identities of the proteins are indicated. (B) A comparison of the ATPase activity stimulated by DNA, histones or nucleosomes of equal amounts of Mi2 alone (rMi2) or in a complex (NuRD). (C) Quantification of the results shown in (B). Error bars represent the average deviation of two independent experiments.
