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Mi2 is an ATP-dependent nucleosome remodeling factor. Mononucleosome disruption assay comparing the efficiency of rMi2 (lanes 2–7) with the NuRD complex (lanes 8–10). End-labeled mononucleosomes with a concentration of 20 nM were incubated with increasing concentrations of rMi2 as indicated, followed by DNase I digestion. The presence or absence of ATP in the reactions is indicated. The concentration of NuRD in the reactions is also indicated.
