Table 1. Kinetic parameters of different nucleosome remodeling factors in ATP hydrolysis.
| Complex |
Cofactor |
Km (µM) |
Vmax (no.
ATP/min/complex) |
References |
| NuRD | DNA | 94 | 1111 | This work |
| Nucleosome | 112 | 4180 | This work | |
| rMi2 | DNA | 30 | 37 | This work |
| Nucleosome | 38 | 189 | This work | |
| xMi2 complex | Nucleosome | ND | <1 | 22 |
| ySWI/SNF | DNA | 100 ± 50 | 1000 ± 200 | 23 |
| Nucleosome | 100 ± 50 | 1000 ± 200 | 23 | |
| yRSC | DNA | 100 ± 50 | 1000 ± 200 | 23 |
| Nucleosome | 100 ± 50 | 1000 ± 200 | 23 |
rMi2, human recombinant Mi2; xMi2 complex, Xenopus Mi2 complex; no., number of ATP molecules hydrolysed in 1 min by each NuRD complex; ND, not determined.