Skip to main content
. 2017 May 17;46(2):1751–1757. doi: 10.1111/ejn.13597

Figure 1.

Figure 1

Myosin II is necessary for AIS plasticity. (A) Myosin II is necessary for 48 h AIS relocation. Example maximum intensity projection images (left) of dentate granule cells labelled for ankyrin‐G (AnkG) and prox1 (Px1) following 48 h NaCl or KCl treatment in the presence of DMSO or 50 μm blebbistatin. Plot (right) shows mean ± SEM of AIS start (S), max (M) and end (E) position for each treatment group. AMI, AIS movement index (see Methods). (B) Myosin II is necessary for 3 h AIS shortening. Example maximum intensity projection images (left) of dentate granule cells labelled for ankyrin‐G (AnkG) and prox1 (Px1) following 3 hr NaCl or KCl treatment in the presence of DMSO or 50 μm blebbistatin. AISL, AIS length. Plot (right) shows cumulative fraction and (inset) mean ± SEM of AIS length. Two‐way anova with Tukey's multiple comparison test; **, P = 0.0054; ns, non‐significant. For example images in (A) and (B), Yellow lines, axon start; white arrowheads, AIS start and end positions; scale bar, 10 μm. [Colour figure can be viewed at wileyonlinelibrary.com].