Figure 3.

Platelet adhesion to immobilized S. gordonii strains under static conditions. Bacterial cells were deposited onto microwells, and platelet binding was determined by phosphatase assay as described in 4. Figures in parentheses indicate ng ml⁻¹ nisin added to cultures to induce expression of PadA or Hsa in the complemented strains UB2775 ΔpadA Δhsa/pMSP‐padA, and UB2777 ΔpadA Δhsa/pMSP‐hsa. Fibrinogen 100 μg ml⁻¹ positive control; BSA 100 μg ml⁻¹ negative control. Error bars represent ±SEM from four independent experiments (n = 4). * P < 0.05