FIG 2.

DVL overexpression rescues the Wnt3a response in DVL KO HEK293 cells. (A to F) Cells were transfected with the indicated combinations of plasmids (the concentration of each plasmid carrying DVL was 10 ng/sample, if not stated otherwise) and on the next day were treated with control (ø) or Wnt3a (3a) conditioned medium (CM) for 2 h (for Western blotting [WB]) or 14 h (for the TopFlash reporter assay). The response to Wnt3a was monitored by WB (S1490-LRP6) (Ai to Di; n = 3) and the TopFlash reporter system (n = 5 for panel E; n = 4 for panel F). pS1490-LRP6 signals were quantified by densitometry and normalized to those for actin (Aii to Dii; n = 3). (Aiii) Transfection efficiency was monitored by cotransfection of GFP. Analysis for statistically significant differences was performed by paired Student's t test (*, P < 0.05; **, P < 0.01).