FIG 10.

Prion-like infectivity of p53 amyloids. Haploid recipient and donor cells of the indicated genotypes were mated, where donor cells were with (+Seed) or without (−Seed) seeds. Following heterokaryon formation, the cytoductants carrying recipient nuclei and a mixture of donor and recipient cytoplasm were selected based on the recipient nuclear genotype and growth on glycerol medium. The resultant cytoductants were transferred onto a YPD plate to assay ADE2 reporter (A) and onto a His–3-AT plate to assay HIS3 reporter (B).