FIG 4.

Effect of p53 expression on yeast growth. (A) Growth curves of the yeast strain with and without GAL-p53 vector and with and without seeds on liquid minimal medium in the absence (left) or presence (right) of galactose. The A₆₀₀ was measured every 2 h. The data points represent the means of the results of three independent experiments. The error bars indicate standard deviations from the mean. (B) Analysis of cell death induced in yeast. Addition of the seeds drastically reduced the percentage of cells stained with the death markers annexin V and PI. Scale bar, ∼5 μm.