FIG 9.

Dominant character and non-Mendelian segregation by p53 amyloids. (A) Haploid pGALp53LacZ (+ Seed) and pADHp53ADE2 (− Seed) haploids were streaked on a YPD plate. The two strains were mated to obtain the desired combination. The resultant strain was streaked on YPD to check the colony assay, which was observed to be plus-seed cells, suggesting the presence of a dominant trait. (B) (Right) p53 amyloids in a diploid cell obtained by mating between the plus-seed and the minus-seed cells displayed non-Mendelian segregation, since all the meiotic spores of almost all the dissected tetrads displayed nonfunctional and aggregated p53, as shown by white colonies. (Left) Mating between the minus-seed cells (control) showing all the meiotic spores carrying functional p53, as shown by blue colonies.